Stem Cell Processing Technician

Organisation:

Location:

Reports to:

1 Month TRAINING in PUNE

About ALIV

Autologous Cell Therapy

Role Summary

process PRP/PRFM

Key Responsibilities

Sample Handling & Chain of Custody

• Receive, label, and track blood and bone-marrow aspirate; maintain time/temperature controls and transport logs.
• Verify consent, requisitions, and kits; ensure traceability from collection to reinfusion.

PRP / PRFM Processing

• Perform anticoagulation, centrifugation per protocol (soft/hard spins), plasma separation, platelet resuspension, PRFM activation.
• Record volumes, spin parameters, platelet yield/concentration, and lot numbers of disposables.

Bone Marrow–Derived Cell Fraction Processing

• Process aspirate using validated

  closed/open systems

  (e.g., RBC depletion, density separation).
• Isolate mononuclear/cell fractions as per SOP; prepare final product for clinical use.

Asepsis, QC & Release

• Work under

  Class II biosafety cabinet/laminar flow

  ; follow sterile technique and PPE.
• Perform in-process QC:

  TNC/platelet counts, viability (e.g., Trypan Blue), recovery %, hemolysis check

  , appearance.
• Prepare batch records and

  Certificate of Processing/Release

  for clinician sign-off.

Equipment & Facility

• Operate and maintain

  centrifuges (fixed/swing), pipettes, cell counters/hemocytometer, vortex, incubators (if applicable)

  .
• Daily/weekly

  calibration, IQ/OQ/PQ logs

  , maintenance tickets, and environmental hygiene records.

Compliance & Safety

• Adhere to

  DCGI/ICMR guidance

  , clinic SOPs, biosafety, biomedical waste rules, and incident/AER reporting.
• Participate in audits, CAPA, and periodic validation runs.

Inventory & Coordination

• Manage stock of

  kits, anticoagulants, reagents, disposables

  ; raise purchase requests proactively.
• Coordinate OR/clinic schedules for timely processing and delivery.

Qualifications

• B.Sc./M.Sc.

  in Biotechnology/Microbiology/Biomedical/Allied Health

  or DMLT/BMLT

  .

• 2–5 years

  in a cell/hematology/transfusion, IVF, orthobiologics, or day-care lab (PRP/BM processing preferred).
• Strong grasp of aseptic technique, centrifugation theory, and basic hematology counts.
• Comfortable with

  EMR/CRM/LIS

  , Google Workspace, and documentation discipline.
• Languages:

  English + Hindi/Marathi (or local)

  .

Skills & Traits

• Meticulous, steady hands, and

  SOP-first

  mindset.
• Data-accurate; converts protocols into repeatable outputs.
• Calm under time pressure; clear communication with clinicians and nursing.
• Integrity, patient privacy, and teamwork.

KPIs

• Yield & viability

  within spec;

  turnaround time

  adherence.

• Documentation accuracy

  (batch records, logs) and SOP compliance.

• Zero contamination/incident rate

  ; successful audits.
• Stockouts avoided; equipment uptime.

90-Day Outcomes

• 30 days:

  Trained and certified on ALIV SOPs; independent PRP runs with complete records.

• 60 days:

  Independent bone-marrow fractionation; QC dashboards live.

• 90 days:

  ≥95% runs within yield/viability specs; audit-ready documentation; proposes at least

  2

  process improvements.