Process R&D - Peptide Chemistry

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1.0 Position Summary

We are seeking a highly skilled and motivated Research Scientist specializing in peptide chemistry. The ideal candidate will be responsible for the design, execution, optimization, and scale-up of synthetic peptides using both Solid-Phase Peptide Synthesis (SPPS) and Liquid-Phase Peptide Synthesis (LPPS) methodologies. This role requires hands-on expertise in various synthesis techniques, purification, and analytical characterization, as well as a strong commitment to safety, documentation, and project management within a dynamic R&D environment.

2.0 Key Responsibilities

A. Peptide Synthesis & Development

• Design and execute multi-step synthesis of complex peptides (up to 30-40 mers) using manual and automated SPPS techniques.
• Demonstrate expertise with a wide range of peptide synthesis resins (e.g., 2-CTC, Rink Amide, Wang, Sieber) and select appropriate resins, protecting groups (Fmoc/Boc), coupling reagents, and solvents for target sequences.
• Perform advanced peptide modifications, including disulfide bridging, lactamization, and other cyclization strategies.
• Conduct peptide synthesis in solution phase (LPPS) utilizing both Fmoc and Boc protection strategies.
• Expertly carry out critical downstream processes, including resin cleavage, global deprotection, quenching, and product isolation.

B. Instrumentation & Technology

• Operate, troubleshoot, and maintain automated peptide synthesizers.
• Utilize microwave peptide synthesizers (e.g., CEM Liberty Blue) to optimize reaction conditions and improve synthesis efficiency.
• Conduct synthesis at various scales using manual peptide reactors (1 L to 10 L).

C. Purification & Analysis

• Purify crude peptides using preparative Reverse-Phase High-Performance Liquid Chromatography (RP−HPLC) and perform subsequent lyophilization.
• Interpret analytical data (e.g., HPLC, Mass Spectrometry) to assess reaction progress, purity, and yield, and generate trend data to guide process optimization.
• Conduct comprehensive literature surveys to support the development of novel and efficient synthesis strategies.

D. Compliance, Safety & Documentation

• Maintain meticulous, real-time documentation of all experiments and results in an Electronic Lab Notebook (eLN).
• Ensure strict adherence to laboratory safety procedures to prevent incidents and maintain a safe working environment.
• Provide technical support for the technology transfer and execution of peptide manufacturing in GMP plant environments.
• Ensure timely calibration and proper functioning of laboratory instruments, including analytical balances and pH meters.

E. Project Management & Collaboration

• Effectively plan and execute projects to ensure timely completion and achievement of R&D milestones.
• Engage in regular interaction and coordination with the Cross-Functional Team (CFT), including Analytical, Quality, and Manufacturing departments.

3.0 Qualifications & Experience

• Education:

  M.Sc. in Organic Chemistry, Medicinal Chemistry, or a closely related field.

• Experience:

  A minimum of 5 to 13 years of direct, hands-on experience in synthetic peptide chemistry within a pharmaceutical or biotechnology industry setting.
• Proven track record in both SPPS and LPPS is highly desirable.
• Experience with process scale-up and knowledge of GMP principles is a strong asset.