Inviting Applications For AR&D positions - HPLC & Prep HPLC

Dear Candidate,

We are Inviting Applications for the positions in our Analytical Research & Development department.

Position Name:

Experience:

Qualification:

Work location:

Key Skills and Competencies:

High-Performance Liquid Chromatography (HPLC)

Key Skills and Competencies:

• Operation and Maintenance

  : Proficiency in operating HPLC systems (e.g., Agilent, Waters, Shimadzu) and performing routine maintenance, calibration, and troubleshooting of equipment issues like pressure fluctuations or detector errors.

• Method Development and Validation

  : Ability to develop and optimize HPLC methods for analyzing small molecules, impurities, or degradation products, adhering to ICH guidelines (e.g., ICH Q2 for validation).

• Data Analysis

  : Expertise in interpreting chromatograms, quantifying analytes, and using software like Empower or ChemStation for data processing and reporting.

• Sample Preparation

  : Skilled in preparing samples (e.g., dissolution, extraction) for pharmaceutical or chemical analysis, ensuring reproducibility and accuracy.

• Regulatory Compliance

  : Knowledge of Good Manufacturing Practices (GMP) and Good Laboratory Practices (GLP) for documentation, method validation, and audit readiness.

• Troubleshooting

  : Ability to diagnose and resolve issues such as peak tailing, baseline drift, or column degradation.

Experience Level (1-5 Years)

• 1-2 years: Basic operation, routine analysis, and data interpretation under supervision.
• 3-5 years: Independent method development, validation, and troubleshooting with familiarity in handling complex matrices.

Prep HPLC

Key Skills and Competencies

Operation and Maintenance

• Hands-on experience with

  Preparative HPLC systems

  (e.g., Agilent, Waters, Shimadzu, GE KTA).
• Proficiency in system setup, gradient programming, scale-up from analytical to preparative runs.
• Routine

  maintenance and calibration

  of pumps, fraction collectors, and detectors.
• Troubleshooting common issues such as pressure fluctuations, air bubbles, or pump leakage.

Purification & Method Development

• Strong background in

  method development and optimization

  for preparative HPLC to isolate target compounds (APIs, impurities, natural products, metabolites, peptides).
• Experience in

  scaling up analytical methods to preparative scale

  while ensuring recovery, purity, and yield requirements.
• Knowledge of different stationary phases (C18, C8, size-exclusion, chiral) and selection of appropriate mobile phases.

Sample Preparation & Fraction Collection

• Skilled in preparing

  crude samples

  (reaction mixtures, fermentation broths, plant extracts) for scalable purification.
• Competence in managing

  fraction collection strategies

  , solvent recovery, and sample lyophilization/drying.
• Ensuring high-quality isolation of compounds with reproducibility.

Data Analysis & Reporting

• Ability to interpret

  chromatograms

  , verify purity and recovery using both

  analytical HPLC/UPLC

  and complementary techniques (LC-MS, NMR).
• Proficiency in software like ChemStation, Empower, Unicorn, or equivalent for

  processing preparative runs

  and reporting results.
• Documentation of purification workflows in compliance with client and regulatory requirements.

Regulatory Compliance & CRO Environment

• Knowledge of

  Good Laboratory Practices (GLP)

  and

  CRO industry requirements

  for method documentation, record-keeping, and reproducibility.
• Familiarity with

  client-driven project timelines

  and requirements.
• Experience preparing project reports, tech-transfer documents, and audit-ready records.

Troubleshooting & Continuous Improvement

• Expertise in diagnosing issues like

  sample overload, poor recovery, carry-over, baseline instability,

  or column degradation.
• Ability to suggest process improvements for higher throughput and purity levels.

Experience Level (Typical Range: 1-5 Years)

• 1-2 years:

• Basic operation of preparative HPLC systems.
• Performing routine purification runs under supervision.
• Basic troubleshooting and hands-on sample preparation.

• 3-5 years:

• Independent purification and

  method development

  for wide compound classes.
• Competent in

  scale-up

  from analytical to preparative levels.
• Handling of challenging crude matrices and optimization of recovery/yield.
• Active participation in

  client projects

  , troubleshooting, and mentoring junior team members.

Interested candidates can contact 6309995773 or share their profiles to