Upstream scientist

Job Title: Protein Expression Scientist

Job Location: Bangalore

Department: Discovery Biology

About Syngene:

Incorporated in 1993, Syngene International Ltd. is an innovation-focused global discovery, development and manufacturing organization providing integrated scientific services to the pharmaceutical, biotechnology, nutrition, animal health, consumer goods and specialty chemical industries around the world. Syngene s clientele includes world leaders such as Bristol-Myers Squibb, Baxter, Amgen, GSK, Merck KGaA and Herbalife. Its innovative culture is driven by the passion of its 4240- strong team of scientists who work with clients from around the world to solve their scientific problems, improve R&D productivity, speed up time to market and lower the cost of innovation.

Key Responsibilities:

• Maintenance of Cell culture, Storage of cells,


• Independently perform experiments for protein expression-Transient transfections, Stable cell line generation, Insect cell expression, Expression in E. coli


• Perform small scale purifications of affinity tag proteins.


• Perform analysis of proteins by SDS-PAGE, Western Blot.


• Troubleshoot issues with protein expression and purification.


• Regularly prepare detailed records of experiments, reports.


• Manage multiple projects simultaneously under strict deadlines.


• Help other team members in their work.


• Contribute to general lab cleanliness, equipment maintenance, Consumable ordering and safety.

Educational Qualification:

• MSc (Biochemistry, Biotechnology or any other relevant Biological Sciences) with 9-12 years of experience in Mammalian Cell based Expression systems and microbial expression systems.
  
  

Experience:

• Experience in performing transfections in Mammalian cells in Shake flask


• Experience in performing microbial expression


• Experience with Protein analysis methods (SDS PAGE, Western).


• Reasonable theoretical knowledge of Proteins, Biochemistry, Cell biology, Mammalian Protein expression, Microbial expression and affinity protein purification.


• Working knowledge of making buffers and solutions, calculations of molarity, dilutions, measuring pH of solutions.


• Good verbal communication, written communication and interpersonal skills


• Ability to manage multiple projects in parallel.


• Ability to work under strict timelines.


• Ability to properly document results and observations in timely manner


• Personal attributes organized, motivated, cooperative, friendly, respectful and communicative.

Behavioral Skills:

• Demonstrate ability to be a team player.


• Commitment to deliver the study reports within the agreed timelines


• Adaptability to changes in the dynamic lab environment


• Communicate confidently with colleagues and collaborators in the meetings and presentation of study reports and evidence of publications in the PEER reviewed Journals

Equal Opportunity Employer: