SCIENTIST

Key Responsibilities

• Lead and execute protein expression and purification projects according to client specifications and timelines.
• Utilize expertise in protein expression systems to optimize production yields and quality.
• Perform protein purification using AKTA FPLC systems and Unicorn control software, with proficiency in automating standard purification processes.
• Develop and validate purification methods to meet project requirements, ensuring high purity and yield of target proteins.
• Conduct analytical characterization of purified proteins using various techniques such as SDS-PAGE, Western blotting, and HPLC.
• Implement aseptic techniques for handling mammalian cell cultures, ensuring sterility and integrity throughout the purification process.
• A thorough understanding of bacterial endotoxin limits and strategies for their elimination from purified proteins.
• Collaborate effectively with cross-functional teams to troubleshoot and solve technical challenges.
• Mentor junior team members, providing guidance and support to foster their professional development.
• Contribute to the continuous improvement of laboratory protocols, processes, and workflows to enhance efficiency and quality.
  

Qualifications

• Master's degree (MSc. or M.Tech) in biotechnology, biology, or a related field. Ph.D. not required.
• 7 to 9 years of hands-on experience in protein expression and purification within an industrial setting, preferably in a contract research organization.
• Proficiency in protein expression systems such as bacterial, yeast, and mammalian cell cultures.
• Extensive experience with AKTA FPLC systems and Unicorn control software, including the ability to automate standard purification processes.
• Strong understanding of protein purification techniques and analytical methods.
• Familiarity with bacterial endotoxin limits and strategies for endotoxin removal.
• Excellent communication skills and ability to work collaboratively in a dynamic team environment.
• Demonstrated ability of proactive and problem-solving mindset.
• Ability to effectively prioritize and manage multiple projects simultaneously while meeting deadlines.