Molecular & Cell Biologist

Molecular Biologist

Key Responsibilities:

• Perform

  DNA and RNA extraction

  and

  plasmid extraction

  at various scales.
• Execute

  vector transformation

  in bacterial strains such as DH5-Alpha or similar.
• Apply a wide range of

  PCR techniques

  including inverse, touch down/up, gradient, long amplicons, TaqMan, and real-time PCR for various applications.
• Carry out

  cloning

  via methods like cut-and-paste, Gibson assembly, Gateway, and In-Fusion.
• Handle, maintain, and stock various

  cell lines

  , particularly HEK 293T, HeLa, MCF-7, and suspension cell lines such as Expi and FreeStyle series.
• Perform

  transfection

  using both chemical and electroporation methods at varying scales.
• Achieve

  protein expression

  in

  bacteria

  ,

  yeast

  ,

  insect

  , and

  mammalian cells

  , and support protein separation and purification (optional).
• Conduct

  protein estimation assays

  (e.g., Bradford, BCA, or ELISA).
• Use

  Western blotting

  for estimating relative protein expression levels.
• Utilize

  FPLC systems

  such as Cytiva's Akta series or Bio-Rad's NGC line (desirable but not mandatory).
• Collaborate with cross-functional teams to advance various R&D projects in mRNA vaccine development, cell line generation, and protein production.

Qualifications:

• PhD

  in Molecular Biology, Cell Biology, or a related field;

  OR

  a

  Master's degree

  in a relevant field with at least

  3+ years of additional experience

  in a relevant laboratory environment.

• Hands-on experience

  with

  DNA and RNA extraction

  , as well as

  plasmid extraction

  at various scales.
• Extensive expertise in

  PCR techniques

  , including real-time PCR and TaqMan assays, for various molecular biology applications.
• Proficiency in

  cloning methods

  , including cut-and-paste, Gibson assembly, Gateway, and In-Fusion.

• Experience with cell line maintenance

  (especially HEK 293T, HeLa, and MCF-7) and

  suspension cell lines

  like Expi and FreeStyle.
• Expertise in

  transfection techniques

  , including chemical and electroporation methods.
• Demonstrated ability to achieve

  protein expression

  in

  bacteria

  ,

  yeast

  ,

  insect

  , and

  mammalian cells

  .
• Experience in

  protein separation and purification

  methods (e.g., FPLC).
• Familiarity with

  protein estimation assays

  like Bradford, BCA, or ELISA, and knowledge of

  Western blotting

  for protein expression analysis.
• Experience with

  FPLC systems

  (Cytiva's Akta or Bio-Rad's NGC series) is a plus but not mandatory.
• Strong ability to

  work independently

  and within a team environment, effectively managing experiments and troubleshooting when necessary.

Preferred Experience:

• Experience with

  mRNA IVT

  (in vitro transcription) techniques.
• Prior exposure to

  monoclonal antibody (mAb)

  production.
• Familiarity with

  cell banking

  and

  stable cell line generation

  .
• Experience working in a

  cGMP

  or regulated environment is a plus.
• A track record of

  publishing scientific findings

  and presenting them clearly in both written and oral formats.

What We Offer:

• Competitive compensation

  and

  benefits package

  .

• Comprehensive health insurance

  , covering immediate family (including pre-existing conditions).
• A

  collaborative, innovative work environment

  focused on solving cutting-edge multidisciplinary challenges in the mRNA vaccine space.

• Career development

  opportunities in a rapidly expanding biotech company.
• A chance to make a

  meaningful impact

  on global health through innovative vaccine development.