Associate Scientist - R&D Cell Line Development

Roles and Responsibilities:

• Perform molecular biology activities including:
  
• Cloning of gene of interest in plasmids for platform processes
  
• In-house vector development
  
• cDNA synthesis
  
• Plasmid preparation
  
• Handling of bacterial cultures and buffer preparations
  
• Genetic engineering activities with minimal supervision
  
• Carry out cell culture-based activities such as:
  
• Transfection, minipool development, and single-cell cloning
  
• Clone screening and characterisation
  
• Media optimisation for production
  
• Early product characterisation as per cell line development plans
  
• Utilisation of platform-based and state-of-the-art technologies
  
• Develop and optimise new methods/assays to support:
  
• Clone development and characterisation
  
• Molecular biology and protein expression studies
  
• Improved assay techniques for R&D functions
  
• Manage day-to-day laboratory operations:
  
• Maintain lab inventory and consumables
  
• Oversee equipment calibration and preventive maintenance
  
• Ensure documentation of lab activities in accordance with GLP (Good Laboratory Practices) and GDP (Good Documentation Practices)
  
• Record experimental data in lab notebooks (LNBs) and review regularly
  
• Create and review function-specific Standard Operating Procedures (SOPs):
  
• Ensure adherence to SOPs across all activities
  
• Support compliance and audit-readiness of the lab
  

Skills Required:

• Strong knowledge and hands-on experience in:
  
• Molecular biology techniques (gene cloning, cDNA synthesis, vector handling)
  
• Mammalian cell culture and stable cell line development
  
• Transfection techniques and clone selection workflows
  
• Assay development and protein biology methods
  
• Good understanding of GLP, GDP, and SOP compliance requirements
  
• Experience or familiarity with

  small-scale bioreactor systems

  , such as

  ambr15

  and

  ambr250

  , is desirable
• Strong documentation, problem-solving, and communication skills
  
• Ability to work independently and as part of a collaborative R&D team