Scientist/Senior Scientist | Molecular Biology

As a Scientist at PopVax, an Indian full-stack biotechnology company, your role will involve executing molecular biology workflows for gene expression, protein production, and assay development for various programs. You are expected to have strong expertise in recombinant DNA technology, experimental design, and troubleshooting. Moreover, you should possess the ability to independently plan and execute projects in a collaborative research environment. Key Responsibilities: - Perform DNA, RNA, and protein extraction, purification, and quantification using established and advanced molecular biology methods. - Design and conduct PCR, qRT-PCR, and cloning workflows using techniques such as restriction enzyme-based, Gibson Assembly, Gateway, Golden Gate, and In-Fusion. - Build vectors from scratch in silico, utilizing molecular design software for sequence optimization and construct validation. - Express recombinant proteins in bacterial, yeast, insect, and mammalian cells, including both transient and stable transfection systems. - Conduct biochemical and molecular assays, including ELISA and nucleic acid/protein quantification using spectroscopic and fluorometric methods. - Perform protein-protein interaction studies (e.g., pull-downs, co-IP, affinity purification) and support assay development for new molecular platforms. - Participate in high-throughput cloning, screening, and expression studies to evaluate construct performance and optimize conditions. Qualifications: - PhD in Biochemistry, Biotechnology, or Molecular Biology, or M.Sc. with 5-7 years of relevant industrial experience in Molecular Biology & protein expression. - Demonstrable experience in molecular biology methods including DNA, RNA, and protein extraction, purification, and quantification. - Experience in PCR, qRT-PCR, and cloning workflows using various techniques. - Proficiency in building vectors from scratch in silico and expressing recombinant proteins in different cell types. - Ability to conduct biochemical and molecular assays, as well as protein-protein interaction studies. - Excellent verbal and written communication skills for presenting scientific findings clearly and concisely. - Willingness to handle substantial assay workloads, troubleshoot, and master new experimental techniques. - Strong planning and execution skills to maximize contribution to assigned projects. - Adherence to good lab practices, maintenance of laboratory records, and compliance with quality standards and regulations. At PopVax, you will benefit from competitive compensation, comprehensive health insurance, and a collaborative work environment focused on addressing the cutting-edge challenges of the novel mRNA platform.,