Scientist - Molecular Biology

Job Title: Sanger Sequencing Specialist

Location:

Role Overview:

Sequencing Specialist

Key Responsibilities:

• Perform

  Sanger sequencing workflows

  , including PCR amplification, purification, and capillary electrophoresis etc.
• Prepare SOPs, engage in Sequencing method development, Operate and maintain sequencing instruments (e.g., ABI 3730xl for Sanger; Illumina or similar platforms for NGS).
• Ability to prepare

  NGS libraries

  for various applications (DNA, RNA, targeted panels, amplicon sequencing) for outsourcing.
• Perform

  quality control

  checks on libraries and sequencing data using tools like Bioanalyzer, Qubit, and qPCR.
• Analyze sequencing data:
• For Sanger: Interpret chromatograms, base calling, and sequence alignment.
• For NGS: Perform primary and secondary analysis (FASTQ QC, alignment, variant calling).
• Troubleshoot technical issues and optimize protocols for improved efficiency and accuracy.
• Maintain detailed documentation of experiments and results in compliance with laboratory standards.
• Collaborate with cross-functional teams to support molecular biology and genomics projects.
• Ensure adherence to

  quality control

  and

  safety guidelines

  in the laboratory.
• Participating in various laboratory operations and software development for sequencing requirements.
• End-to-end responsibility of maintaining the sequencing facility but not limited to ordering reagents & spare parts, calibration, PM, repair, training etc.

Qualifications & Skills:

Education:

• Experience:

• 4-6 years of hands-on experience in

  Sanger sequencing

  and

  similar workflows

  .
• Experience in NGS library preparation and sequencing platforms (Illumina, Ion Torrent, etc.) is a plus.
• Familiarity with sequencing analysis software and bioinformatics tools (e.g., Chromas, Sequencing Analysis, FASTQC, BWA, GATK etc.).
• Strong understanding of PCR, primer design, and DNA/RNA purification techniques and equipment.
• Excellent troubleshooting and problem-solving skills.
• Ability to work independently and manage multiple projects simultaneously.
• Good communication and documentation skills is a must.

Preferred:

• Experience with

  high-throughput sequencing

  and automation platforms.
• Knowledge of

  advanced bioinformatics pipelines

  for variant analysis and annotation.

Key competencies: